PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
SpecificityRad2/FEN1 (Phospho-Ser187) Antibody detects endogenous levels of Rad2/FEN1 only when phosphorylated at Ser187
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human Rad2/FEN1 (Phospho-Ser187)
Target NameRad2/FEN1
ModificationPhospho-Ser187
Alternative NamesFEN1, DNase IV, FEN-1, HFEN-1, Flap endonuclease 1, MF1, RAD2, Maturation factor 1, Maturation factor-1