PurificationAntibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
ApplicationsWB
Species ReactivityHu Ms Rt
Specificityp70 S6 Kinase (Phospho-Ser427) Antibody detects endogenous levels of p70 S6 Kinase only when phosphorylated at Ser427
Immunogen TypePeptide
Immunogen DescriptionA synthesized peptide derived from human p70 S6 Kinase (Phospho-Ser427)