EGFR(Phospho-Thr693) Antibody

Description

Product Name EGFR(Phospho-Thr693) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level EGFR only when phosphorylated at threonine 693.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 693 (P-L-T(p)-P-S) derived from Human EGFR.
Target Name EGFR
Modification Phospho-Thr693
Alternative Names ERBB1; Receptor protein-tyrosine kinase ErbB-1; kinase EGFR
Accession No. Swiss-Prot: P00533
NCBI Protein: NP_005219.2
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 175kd
Western blotting: 1:500~1:1000

Images

Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Thr693) Antibody #AB11187.
Western blot analysis of extracts from HUVEC cells, treated with starve or calf intestinal phosphatase (CIP), using EGFR (Phospho-Thr693) Antibody #AB11187.

Background

Receptor for EGF, but also for other members of the EGF family, as TGF-a, amphiregulin, betacellulin, heparin-binding EGF-like growth factor, GP30 and vaccinia virus growth factor. Is involved in the control of cell growth and differentiation. Phosphorylates MUC1 in breast cancer cells and increases the interaction of MUC1 with SRC and CTNNB1/beta-catenin.

Doherty JK, et al. (1999) Proc Natl Acad Sci U S A; 96(19): 10869-10874
Wu TT, et al. (1998) Mol Biol Cell; 9(7): 1661-1674
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