Human IL-17 ELISA kit

Description

Product Name Human IL-17 ELISA kit
Brief Description ELISA Kit
Applications Solid Phase Sandwich ELISA
Species Reactivity Human
Specificity Natural and recombinant Human IL-17 Ligand
Crossing Reactivity No significant interference observed with available related molecules.
Target Name Human IL-17

Application Details

Detect Range: 15.625-1000pg/ml
Sensitivity: 7pg/mL
Sample Type: Cell culture supernatant, serum, plasma (EDTA, citrate, heparin)
Sample Volume: 20 uL
Assay Time: 3 hours
Detection method: Colorimetric

Images

Representative standard curve for IL-17 ELISA. IL-17 was diluted in serial two-fold steps in Sample Diluent.

Product Description

Aluminium pouches with a Microwell Plate coated with antibody to human IL-17 (8x12)
1 vials human IL-17 Standard lyophilized,1000 pg/ml upon reconstitution
1 vials concentrated Biotin-Conjugate anti-human IL-17 antibody
1 vials Streptavidin-HRP solution
1 bottle Standard /sample Diluent
1 bottle Biotin-Conjugate antibody Diluent
1 bottle Streptavidin-HRP Diluent
1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
1 vial Substrate Solution
1 vial Stop Solution
2 pieces Adhesive Films
package insert

Background

IL-17, originally identified as mouse cytotoxic T lymphocyte-associated antigen-8 (CTLA-8) (1), is produced by activated T lymphocytes, primarily by memory T cells (1-4). IL-17 appears to mediate communication between the immune system and the hematopoietic system.

IL-17 is a disulfide-linked homodimer (2, 4). Each polypeptide has 155 amino acid (aa) residues (predicted mass = 17.5 kDa), including a 19 aa residue hydrophobic leader sequence (2). There are six cysteines plus one potential N-linked glycosylation site, which is variably glycosylated, at least with recombinant proteins (2, 4). The aa sequence of human IL-17 is 63% and 58% identical to mouse and rat IL-17 and 72% identical to the thirteenth ORF of Herpesvirus saimiri (2, 4). There is at least some species specificity for in vitro action on bone-marrow stromal cells (3).

IL-17 mediation of T cell communication with the hematopoietic system is suggested by two observations. T cell-derived IL-17 induces fibroblasts to produce IL-6, IL-8, ICAM-1 and G-CSF, apparently by an NF-?B-mediated mechanism (5). IL-6 in turn promotes development of granulocyte/macrophage colonies, and G-CSF directs development of neutrophils (4, 6-9). IL-17 also enhances proliferation of partially activated T cells (5) and upregulates nitric oxide (NO) production in osteoarthritic cartilage (10).

Regerences

Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Yao, Z. et al. (1995) J. Immunol. 155:5483.

Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.

Fossiez, F. et al. (1996) J. Exp. Med. 183:2593.

Yao, Z. et al. (1995) Immunity 3:811.

Ikebuchi, K. et al. (1987) Proc. Natl. Acad. Sci. USA 84:9035.

Berliner, N. et al. (1995) Blood 85:799.

Roberts, A.W. and D. Metcalf (1994) Exp. Hematol. 22:1156.

Broxmeyer, H.E. (1996) J. Exp. Med. 183:2411.

Attur, M.G. et al. (1997) Arthritis Rheum. 40:1050.

Published Papers