Human IL-8 ELISA kitEK0453

• Aluminium pouches with a Microwell Plate coated with antibody to human IL-8 (8 x12)
• 1 vials human IL-8 Standard lyophilized, 2000 pg/ml upon reconstitution
• 1 vials concentrated Biotin-Conjugate anti-human IL-8 antibody
• 1 vials Streptavidin-HRP solution
• 1 bottle Standard /sample Diluent
• 1 bottle Biotin-Conjugate antibody Diluent
• 1 bottle Streptavidin-HRP Diluent
• 1 bottle Wash Buffer Concentrate 20x (PBS with 1% Tween-20)
• 1 vial Substrate Solution
• 1 vial Stop Solution
• 2 pieces Adhesive Films
• package insert

Interleukin 8 (IL-8), a member of the neutrophil-specific CXC subfamily of chemokines, is a potent neutrophil chemotactic and activating factor. It is a primary inflammatory cytokine produced by many cells including monocytes/ macrophages, T cells, neutrophils, fibroblasts, endothelial cells, keratinocytes, hepatocytes, astrocytes and chondrocytes. It is in response to proinflammatory stimuli such as IL-1, TNF, LPS and viruses. Its function is, in part, to attract neutrophils to the site of inflammation and to activate them(1-6).

The human IL-8 cDNA sequence predicts a protein of 99 amino acids. Removal of a 22-residue signal peptide generates a mature protein of 77 amino acids (~ 8 kDa). Further proteolysis of the N-terminal end leads to a variant form with 72 amino acids; full activation of IL-8 may require cleavage to the 72 amino acid form.

IL-8 can form non-covalent dimers in solution, especially at high concentrations, but dimerization is not necessary for biological activity. IL-8 binds to two seven-transmembrane, G protein-coupled receptors, CXCR1 and CXCR2, as well as to the non-signalling Duffy antigen on red-blood cells. The Duffy antigen may play a role in regulating IL-8 activity on functional receptors.

Oppenheim, J.J. (1991) Annu. Rev. Immunol. 9:617.

Miller, M.D. and M.S. Krangel (1992) Crit. Rev. Immunol. 12:17.

Matsushima, K. et al. (1992) Chem. Immunol. 51:236.

Taub, D.D. and J.J. Oppenheim (1993) Cytokine 5:175.

Vaddi, K. et al. (1997) The Chemokine Facts Book, Academic Press, p. 23.

Hack, C.E. et al. (1997) Adv. Immunol. 66:101.