LIMK1(Phospho-Thr508) Antibody

Description

Product Name LIMK1(Phospho-Thr508) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IHC IF
Species Reactivity Hu Ms Rt
Specificity The antibody detects endogenous level of LIMK1 only when phosphorylated at threonine 508.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of threonine 508 (R-Y-T(p)-V-V) derived from Human LIMK1.
Target Name LIMK1
Modification Phospho-Thr508
Alternative Names LIMK-1; kinase LIMK1;
Accession No. Swiss-Prot: P53667
NCBI Protein: NP_002305.1
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 72kd
Western blotting: 1:500~1:1000
Immunohistochemistry: 1:50~1:100
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from UV-treated C2C12, PMA-treated Hela and anisomycin-treated 293 cells using LIMK1(Phospho-Thr508) Antibody #AB11126.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using LIMK1(Phospho-Thr508) Antibody #AB11126(left) or the same antibody preincubated with blocking peptide(right).
Immunofluorescence staining of methanol-fixed Hela cells using LIMK1(Phospho-Thr508) Antibody #AB11126.
Lane 1 : Hela treated with PMA; Lane 2 : Mouse brain tissue; Lane 3 : Rat brain tissue; Lane 4 : N-peptide blocked Hela treated with PMA; Lane 5 : N-peptide blocked Mouse brain tissue; Lane 6 : N-peptide blocked Rat brain tissue; Lane 7 : P-peptide blocked Hela treated with PMA; Lane 8 : P-peptide blocked Mouse brain tissue; Lane 9 : P-peptide blocked Rat brain tissue; Lysates/proteins at 40 µg per lane. Predicted band size : 72 kDa Observed band size : 72 kDa

Background

Protein kinase which regulates actin filament dynamics. Phosphorylates and inactivates the actin binding/depolymerizing factor cofilin, thereby stabilizing the actin cytoskeleton. Isoform 3 has a dominant negative effect on actin cytoskeletal changes. May be involved in brain development.

Soosairajah J, et al. (2005) EMBO J.
Ohashi K, et al. (2000) J Biol Chem; 275(5): 3577-82.
Edwards DC, et al. (1999) Nat Cell Biol; 1(5): 253-9.

Published Papers

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