p53(Phospho-Ser33) Antibody

Description

Product Name p53(Phospho-Ser33) Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy using non-phosphopeptide.
Applications WB IF
Species Reactivity Hu
Specificity The antibody detects endogenous level of p53 only when phosphorylated at serine 33.
Immunogen Type Peptide-KLH
Immunogen Description Peptide sequence around phosphorylation site of serine 33 (V-L-S(p)-P-L) derived from Human p53.
Target Name p53
Modification Phospho-Ser33
Alternative Names P53, BCC7, LFS1, TRP53
Accession No. Swiss-Prot: P04637
NCBI Protein: NP_000537.3
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20°C for long term preservation (recommended). Store at 4°C for short term use.

Application Details

Predicted MW: 53kd
Western blotting: 1:500~1:1000
Immunofluorescence: 1:100~1:200

Images

Western blot analysis of extracts from HT29 cells using p53(Phospho-Ser33) Antibody #AB11097 and the same antibody preincubated with blocking peptide.
Immunofluorescence staining of methanol-fixed Hela cells using p53(Phospho-Ser33) Antibody #AB11097.
Western blot analysis of extracts from HT29 cells, treated with serum or calf intestinal phosphatase (CIP), using p53 (Phospho-Ser33) Antibody #AB11097.

Background

Acts as a tumor suppressor in many tumor types; induces growth arrest or apoptosis depending on the physiological circumstances and cell type. Involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. One of the activated genes is an inhibitor of cyclin-dependent kinases. Apoptosis induction seems to be mediated either by stimulation of BAX and FAS antigen expression, or by repression of Bcl-2 expression. Implicated in Notch signaling cross-over.

Lin T, et al. (2005) Nat Cell Biol; 7(2): 165-71.
Vega FM, et al. (2004) Mol Cell Biol; 24(23): 10366-80.
Li J, et al. (2004) J Biol Chem; 279(40): 41275-9.
Wang J, et al. (2004) J Biol Chem; 279(38): 39584-92.

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