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GST-Tag antibodyABT528

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Availability: The lead time is currently 2 weeks.

Description

Product Name GST-Tag antibody
Host Species Rabbit
Clonality Polyclonal
Purification Antibodies were purified by affinity purification using immunogen.
Applications WB
Specificity The antibody detects endogenous level of total GST-Tag antibody.
Immunogen Type Recombinant Protein
Immunogen Description Recombinant protein of human GST-Tag.
Target Name GST-Tag
Concentration 1.0mg/ml
Formulation Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:1000 - 1:2000

Images

Western blot analysis of GST-tagged protein using GST antibody at different dilution. Each lane was loaded with 5 ng protein.

Background

Plasmid vectors for the expression of coding regions of eukaryotic genes in E.coli are in common usage; such expression vectors often encode hybrid fusion proteins containing part prokaryotic and part eukaryotic specified proteins. Forinstance, the pGEX.3X expression vector developed by Smith and Johnson allows for synthesis of fusion proteins between glutathionine-S-transferase(GST) and proteins encoded by inserted cDNA sequences. Antibodies derived from these GST fusion proteins are useful for checking protein expression bothin plaques and on Western blots as well as for immunoaffinity purification of proteins expressed in E. Coli.

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