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GAPDH Rabbit Polyclonal AntibodyAB37985

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Description

Product Name GAPDH Rabbit Polyclonal Antibody
Host Species Rabbit
Clonality Polyclonal
Purification Affinity purification using immunogen.
Applications WB IHC
Species Reactivity Hu Rt Ms Mk Chk Hm Sh Xenopus
Specificity Antibody detects endogenous GAPDH protein.
Target Name GAPDH
Alternative Names G3PD; GAPD; MGC88685
Accession No. Swiss-Prot#:P04406
SDS-PAGE MW 37kd
Concentration 1.0mg/ml
Formulation IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:5000~1:20000

Images

Western blot analysis of 293T (1), Rat brain (2), NIH 3T3(3), Sheep Muscle(4),Rabbit testis(5), using #AB37985 diluted at 1:20,000.

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

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