PurificationAffinity purification using immunogen.
ApplicationsWB
Species ReactivityHu Rt Ms
SpecificityThe β I tubulin antibody can recognize endgenousβ I tubulin proteins.
Target Nameβ I tubulin
SDS-PAGE MW55kd
Concentration1.0mg/ml
FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Images
Western blot analysis of 1) Hela, 2) Mouse Brain Tissue, 3) Rat Brain Tissue, using #AB37983 diluted at 1:5,000.
Background
Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin.