α-tubulin Mouse Monoclonal Antibody

Description

Product Name α-tubulin Mouse Monoclonal Antibody
Host Species Mouse
Clonality Monoclonal
Purification Affinity purification using immunogen.
Applications WB IF IP
Species Reactivity Hu Rt Ms
Specificity The α-tubulin antibody can detects endogenousα-tubulin protein.
Target Name α-tubulin
Alternative Names alpha-tubulin N-acetyltransferase; ATAT1; C6orf134; CF134;
Accession No. Swiss-Prot#:Q71U36/P68363
Gene ID: 7846/10376
SDS-PAGE MW 52kd
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:5000~1:10000
Immunofluorescence: 1:100~1:200
Immunoprecipitation: 1:200

Images

Western blot analysis of 1) Hela, 2) Rat BrianTissue, 3) Mouse Brain Tissue, using #AB37981 diluted at 1:5,000.
IF analysis of?Hela?with #AB37981(Left) and DAPI (Right) diluted at 1:100.
1¡¢Input: Mouse Brain Tissue Lysate
2¡¢IP product: IP dilute 1:200
Western blot analysis: primary antibody : #AB37981 1:5,000
Secondary antibody: Goat anti-Mouse IgG, Light chain specific, 1:5,000

Background

Tubulin is one of several members of a small family of globular proteins. The tubulin superfamily includes five distinct families, the alpha-, beta-, gamma-, delta-, and epsilon-tubulins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins that make up microtubules. Each has a molecular weight of approximately 55 KD. Microtubules are assembled from dimers of α- and β-tubulin

Published Papers

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