Flag-tag Mouse Monoclonal Antibody (2C5)

Description

Product Name Flag-tag Mouse Monoclonal Antibody (2C5)
Host Species Mouse
Clonality Monoclonal
Purification Affinity purification using immunogen.
Applications WB
Species Reactivity N/A
Specificity The Flag tag antibody can recognize C-terminal, internal, and N-terminal Flag-tag fusion?proteins.
Target Name Flag-tag
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:5000~1:10000

Images

Western blot analysis of Recombinant protein, using #ABT524 diluted at 1) 1:5,000, 2) 1:10,000.

Background

The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits.A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.

Published Papers

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