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His-Tag Mouse Monoclonal Antibody (4E6)ABT523

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Description

Product Name His-Tag Mouse Monoclonal Antibody (4E6)
Host Species Mouse
Clonality Monoclonal
Purification Affinity purification using immunogen.
Applications WB
Species Reactivity N/A
Specificity The His tag antibody can recognize C-terminal, internal, and N-terminal His-tag fusion proteins..
Target Name His-Tag
Concentration 1.0mg/ml
Formulation Mouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Storage Store at -20˚C

Application Details

Western blotting: 1:3000~1:10000

Images

Western blot analysis of His recombinant protein, using #ABT523 diluted at 1£©1:10,000, 2£©1:20,000

Background

A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. Monoclonal antibodies specific to six histidine tags can greatly improve the effectiveness of several different kinds of immunoassays, helping researchers identify, detect, and purify polyhistidine fusion proteins in bacteria, insect cells, and mammalian cells.

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