FormulationMouse IgG1 in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 51% glycerol.
StorageStore at -20˚C
Application Details
Western blotting: 1:5000~1:10000
Images
Western blot analysis of Avi-Recombinant protein, using #ABT517 diluted at 1) 1:5,000 2) 1:10,000
Background
The Avi tag is a biotin-acceptor peptide1, GLNDIFEAQKIEWHE. The 15-residue peptide served as a substrate mimic for biotin ligase (BirA), which usually recognizes the much larger protein domain.